Glycochip

Glycoconjugates for Glycobiology

GlycoNZ offers a glycoarray for profiling specificity of lectins and antibodies for scientific research

$500 / glycochip

Analysis of plasma samples for anti-glycan antibodies. High analytical sensitivity and specificity, with a broad dynamic range for titer, avidity and affinity.

Characteristics:

  • 25×75×1 mm glass or plastic slides
  • ~600 immobilized ligands
  • ~400 synthetic mammalian glycans
  • ~200 pathogenic bacteria polysaccharides
  • Specific IgG and IgM measurable
  • Shelf life: 1 year at +4°C
  • Identification barcodes
  • ~ 3 hours analysis time

(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411795/)

Any custom configuration of the chip is possible on request. Specialized thematic arrays, as

  • Blood group antigens
  • Cancer-associated antigens
  • Galectin ligands
  • Siglec ligands
  • Contact for custom design

Glycochip uses:

  • Search for glycan-binding proteins and characterization of their specificity; the proteins are, antibodies, lectins, glycosyltransferases, glycosidases [1-7];
  • Functional revealing of lectins and other carbohydrate-binding and carbohydrate-modifying molecules in composition of bacteria, viruses and cells;
  • Development of anti-bacterial and anti-viral compounds acting on microorganism’s lectins;
  • Search for diagnostic signatures (combination of several antibodies-markers), for cancer, autoimmune and other diseases diagnostics [8-11].

References

  1. P.Obukhova, R.Rieben, N.Bovin. Normal human serum contains high levels of anti-Gala1-4GlcNAc antibodies. Xenotransplantation14, 627-635 (2007). PMID: 17991151.
  2. M.E.Huflejt, M.Vuskovic, D.Vasiliu, H.Xu, P.Obukhova, N.Shilova, A.Tuzikov, O.Galanina, B.Arun, K.Lu, N.Bovin. Anti-carbohydrate antibodies of normal sera: findings, surprises and challenges.Molecular Immunology46, 3037-3049 (2009). PMID: 19608278.
  3. T.Pochechueva, A.Chinarev, M.Spengler, E.Korchagina, V.Heinzelmann-Schwarz, N.Bovin, R.Rieben. Multiplex suspension array for human anti-carbohydrate antibody profiling. Analyst136, 560-569 (2011). PMID: 21107457.
  4. T.Pochechueva, F.Jacob, D.R.Goldstein, M.E.Huflejt, A.Chinarev, R.Caduff, D.Fink, N.Hacker, N.V.Bovin, V.Heinzelmann-Schwarz. Comparison of suspension array, ELISA and printed glycan array in the detection of human anti-glycan antibodies. Glycoconjugate J.8-9, 507-517 (2011). PMID: 21948103.
  5. Biochemistry (Moscow),76, 862-6 (2011). PMID: 21999548.
  6. P.Obukhova, V.Piskarev, V.Severov, G.Pazynina, A.Tuzikov, M.Navakouski, V.Shilova, N.Bovin. Profiling of serum antibodies with printed glycan array: room for data misinterpretation. Glycoconj. J.,8-9, 501-505 (2011). PMID: 22057658.
  7. P.Obukhova, E.Korchagina, S.Henry, N.Bovin. Natural anti-A and anti-B of the ABO system: allo- and auto-antibodies have different epitope specificity. Transfusion52, 860-869 (2011). PMID: 21981750.
  8. N.V.Shilova, M.J.Navakouski, N.Khasbiullina, O.Blixt, N.V.Bovin. Printed glycan array: antibodies as probed in undiluted serum and effects of dilution. Glycoconj. J., 29, 87-91 (2012). PMID: 22258790.
  9. N.Bovin, P.Obukhova, N.Shilova, E.Rapoport, I.Popova, M.Navakouski, C.Unverzagt, M.Vuskovic, M.Huflejt. Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies?Biochim. Biophys. ActaGeneral Subjects1820, 1373-1382 (2012). PMID: 22365885.
  10. Bovin N.V. Natural antibodies to glycans. Biochemistry(Moscow), 78, 786-797 (2013). Published in Russian in Biochemistry (Mosc), 2013, 78, 1008-1022. PMID: 24010841.
  11. N.Shilova, M.E.Huflejt, M.Vuskovic, P.Obukhova, M.Navakouski, N.Khasbiullina, G.Pazynina, O.Galanina, A.Bazhenov, N.Bovin. Natural antibodies against sialoglycans. Top Curr Chem., Sept 14 (2013). PMID: 24037491.