About us

Glycoconjugates for Glycobiology

GlycoNZ is the supplier of Synthaur LLC manufactured products, active in glycobiology and glycotechnology, important and growing fields in life science. The product section contains more than 1300 products including > 900 readily available stock items in 4 variations (BM, BP, FP PA) and > 400 bespoke custom products in the 4 variations and an additional 100 glycoconjugates via the glycochip only, which are the result of many years of experience acquired in the academic world.

The range of products and services we offer is mainly orientated on the study of the functions of carbohydrates, the search and study of carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions.

Due to the great flexibility and reproducibility of our synthetic technology, we are in the position to rationally design and produce new glycoconjugates perfectly suited for each particular application. We already have great experience in collaborative research with pharmaceutical companies and academic institutions. At any time we are ready to help our customers in the design and production of new glycoconjugates, in the development of test-systems for diagnostics and HTP screening, or in the study of the fine specificity of carbohydrate-binding proteins.

We can also perform custom synthesis using substances available at the company or starting from compounds supplied by the customer. We are seeking pharmaceutical and diagnostic partners that can offer a combined range of expertise focusing on innovative areas. We welcome the opportunity to meet with all interested parties to explore mutually beneficial collaborations.

Products

Biotinylated monomeric probes

The probes have an elongated spacer giving them the possibility to bind avidin or streptavidin.

The biotin fragment is hydrophobic enough to permit the probes to be adsorbed on SepPack C18 cartridges from aqueous solutions and to be eluted by methanol similarly to glycosides bearing a “Lemieux-type” space.

The spacer is the normal spacer of the ligand plus own spacer (sp) of biotin label –CO(CH2)5NH-

Biotinylated polymeric probes

PAA is poly[N-(2-hydroxyethyl)acrylamide], used as a high molecular weight carrier, has low non-specific sorption and it is stable to chemical and proteolytic action.

The affinity of the probes is 102 – 105 times higher than that of the corresponding free sugars.

HOCH2CH2NH3+-salts for acidic sugars

Spacer-arm is normally –(CH2)3-, spacer arm for biotin is –(CH2)6-.

The flexible polymer chain behaves as an additional spacer.

Mr approx. 30kDa

Content: saccharide 20% mol., biotin 5% mol.

Purity: synthetic ω-aminoalkyl glycosides used for coupling with the polymer have a purity >95% (HPLC and 1H-NMR)

Fluorescein-labelled polymeric probes

Probes designed to reveal lectins (including selectins) directly on cell membranes, organelles, and in the cytoplasm.

The low fluorescein content does not affect the probe solubility in water but it still allows a reliable probe detection.

PAA used as a molecular weight carrier demonstrated an absence of non-specific interaction with cells and it is stable to chemical and proteolytic action.

The affinity of the probes is 102 – 105 times higher than that of corresponding free sugars.

PAA is poly[N-(2-hydroxyethyl)acrylamide], a flexible polymer chain serves as an additional spacer.

Mr approx. 30kDa

Content: carbohydrate 20% mol.

Unlabelled polyacrylamide conjugates

HOCH2CH2NH3+-salts for carboxylates and sulfates

Spacer-arm for oligosaccharides is normally -(CH2)3

PAA is poly[N-(2-hydroxyethyl)acrylamide], a flexible polymer chain serves as an additional spacer.

Mr approx. 30kDa

Content: carbohydrate 20%

Profiling specificity of lectins and antibodies

Analysis of plasma samples for anti-glycan antibodies. High analytical sensitivity and specificity, with a broad dynamic range for titer, avidity and affinity.

Characteristics:
  • 25×75×1 mm glass or plastic slides
  • ~600 immobilized ligands
  • ~400 synthetic mammalian glycans
  • ~200 pathogenic bacteria polysaccharides
  • Specific IgG and IgM measurable
  • Shelf life: 1 year at +4°C
  • Identification barcodes
  • ~ 3 hours analysis time
  • (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411795/)

Any custom configuration of the chip is possible on request. Specialized thematic arrays, as:

  • Blood group antigens
  • Cancer-associated antigens
  • Galectin ligands
  • Siglec ligands
  • Contact for custom design
Glycochip uses:
  • Search for glycan-binding proteins and characterization of their specificity; the proteins are, antibodies, lectins, glycosyltransferases, glycosidases;
  • The functional revealing of lectins and other carbohydrate-binding and carbohydrate-modifying molecules in the composition of bacteria, viruses and cells;
  • Development of anti-bacterial and anti-viral compounds acting on microorganism’s lectins;
  • Search for diagnostic signatures (a combination of several antibodies-markers), for cancer, autoimmune and other diseases diagnostics

Services

Instant synthesis

Tailored PAA-glycoconjugate:
    • with another label (see list of labels below)
    • with two different labels (e.g. fluor and PE)
    • with two different sugar ligands
    • with custom sugar density (from 5% to 40%)
    • with high MW, 1-2 million Da
    • SETs of sugar-PAA, differing by sugar percentage

– Terms of service: 4 – 8 weeks
– Prices are similar to the corresponding regular conjugates
– Synthesis scale: 4 mg and more

Custom synthesis

Polymeric glycoconjugates
Cluster glycopeptides
Monomeric glycoconjugates:
    • Sug-sp-biot
    • Sug-sp-(fluorescent label)
    • glycolipids for insertion into liposomes & biological membranes
    • others

– Precursors for precursors for labelling with 3H, 14C, I
– Activated substances for attachment to SPR chip and other surfaces
– Custom “glycosylation” of glass, stell, solid and other materials
– Affinity adsorbents for isolation of antibodies, lectins, enzymes
– Affinity adsorption for ex vivo applications

Labels available