Biotinylated Monomeric Probes
The probes have an elongated spacer giving them the possibility to bind avidin or streptavidin.
The biotin fragment is hydrophobic enough to permit the probes to be adsorbed on SepPack C18 cartridges from aqueous solutions and to be eluted by methanol similarly to glycosides bearing a “Lemieux-type” space.
The spacer is the normal spacer of the ligand plus own spacer (sp) of biotin label –CO(CH2)5NH-
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