For more questions, please email GlycoNZ
How do I place an order?
Place an order via email with your purchase order attached to GlycoNZ
Can I order free saccharides?
Usually, free saccharides and aminoalkyl glycosides are not available for sale. Nevertheless, some of them could be available on the custom synthesis principle. Please contact us directly by e-mail: GlycoNZ
What is the composition of conjugates?
We offer the conjugates based on polyacrylamide loaded with 20% mol. of oligosaccharide, as we have found through our experiences that this value is optimal. Carbohydrate probes additionally contain biotin (5% mol.), fluorescein (1% mol.), or another label. The knowledge of the optimal conditions for the condensation reaction gives us the possibility to synthesize conjugates with the exact defined molar content of ligands. The real size of the conjugate was evaluated by electron microscopy: the molecule had an almost perfectly spherical form with the diameter of about 150Å, which agrees well with its hydrodynamic size by indirect methods. For the majority of bioanalytical problems, the molecular mass of conjugates of about 30-50 kDa is sufficient and probably optimal. More information about composition, properties, labels, see in Review “Polyacrylamide-Based Glycoconjugates as Tools in Glycobiology” [Glycoconjugate Journal, 15, 431-446, 1998].
What is OPTI-SET?
We produce a series of unlabelled PAA-based neoglycoconjugates with different contents of carbohydrates (for example 2, 5, 10, 15, 20 and 30% mol.) to optimize studying of their interaction with carbohydrate-binding materials. In case you choose the optimal conjugate for your assay from SET you could order it separately, the price of it shall correspond to regular 20% conjugate of the same ligand. Labelled SETs may be enquired too.
What is the purity of ordered product?
Synthetic w-aminoalkyl glycosides used for coupling with the polymer have a purity of >95% as controlled by HPLC and NMR. The composition of the conjugates is confirmed by acid hydrolysis followed by HPLC of AMC-monosaccharides. We have specifications which describe data analysis, storage and other information. Please note in the order if you wish to receive an enclosed certificate of analysis.
Can I order a specific lot?
You can request a batch lot you have used before, but we cannot guarantee it will still be in stock. When you place the order, please note if you would like to reserve more quantity of certain reagents.
How to keep reagents?
Long-term storage for sialylated, phosphated and sulfated compounds we recommend at – 20°C, for other probes at 4°C.
Concentrated solutions of the conjugates can be stored for several days in glassware at 4°C, for long-term storage, we recommend to lyophilize the solutions again.
Long-term storage of diluted solutions of glycoconjugates in plasticware causes the decrease in its concentration due to adsorption on the vessel surface.
What do you recommend as a negative control?
We offer our customers to use corresponding derivatives of aminoglucitol HOCH2(HOCH)4CH2NH- (ligand #0000) as a negative control. However, in each case you can choose additionally more suitable negative controls, for example during analyses of anti-Ley antibodies specificity you can use Lex -PAA, H(type 2)-PAA and Leb -PAA.
What glycoadsorbents do you have?
- We have adsorbents based on rigid macroporous glass particles (pore diameter 2000Å) or on Sepharose-FF. Both of them have a macroporous structure of the carrier enables its high capacity.
- We have listed here adsorbents corresponding to the products available in the stock list. Adsorbents loaded with other carbohydrate ligands manufactured are available on custom synthesis principle.
- We also are ready to prepare adsorbents from aminoalkyl glycosides, glycopeptides or reducing saccharides provided by the customer.